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KMID : 0043320070300111455
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Archives of Pharmacal Research
2007 Volume.30 No. 11 p.1455 ~ p.1463
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Anti-proliferate and Pro-apoptotic Effects of 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyranone through Inactivation of NF-κB in Human Colon Cancer Cells
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Ban Jung-Ok
Hwang In-Guk
Kim Tae-Myoung
Hwang Bang-Yeon
Lee Ung-Soo
Jeong Heon-Sang
Yun Young-Won
Kim Dae-Joong
Hong Jin-Tae
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Abstract
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Many natural compounds have been shown to prevent cancer cell growth through the redox regulation of transcription factors. NF-κB, a redox transcription factor, has been implicated in the apoptotic cell death of several cancer cells. This study examined whether or nor 2,3-dihydro- 3,5-dihydroxy-6-methyl-4H-pyranone (DDMP) isolated from onions can modulate the activity of NF-κB, thereby induce the apoptotic cell death of colon cancer cells. Treatment with different DDMP concentrations (0.5-1.5 mg/mL) for various periods (0-48 h) inhibited the growth of colon cancer cells (SW620 and HCT116) followed by the induction of apoptosis in a dose dependent manner. It was also found that DDMP modulated tumor necrosis factor-α (TNF-α) and tetradeanoyl phorbol acetate (TPA)-induced NF-κB transcriptional and DNA binding activity. Moreover, DDMP suppressed the NF-κB target anti-apoptotic genes (Bcl-2), whereas it induced the expression of the apoptotic genes (Bax, cleaved caspase-3 and cleaved PARP). These results suggest that DDMP from onions inhibit colon cancer cell growth by inducing apoptotic cell death through the inhibition of NF-κB.
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KEYWORD
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Onion (Allium cepa L.), 2, 3-Dihydro-3, 5-dihydroxy-6-methyl-4H-pyranone, Nuclear transcription factor-κB, SW620, HCT116, Apoptosis
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